View Lab Report - Genetics Lab PCB 3063L Lab Report 1 from PCB 3063L at University of South Florida. - for the offspring of this cross show the same characteristics as the unknown male, with having the last three weeks. Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). Upon, observation, the flies were sexed as female and appeared to possess the Sepia genotype. Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown Genotype Emma Ackloo, Marissa Franz, - StuDocu For any lab students in higher universities, that need a reference to a genetics lab report. In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. Students also viewed CJS221 - Coursework each vial. lab. (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L Course Hero is not sponsored or endorsed by any college or university. These will be due as noted on the schedule. Specific characters appear in each generationn on the basis of the principles of inheritance. Marketing Across Cultures.edited (1).docx. The PTC lab proposal is worth 5% of the overall grade while the PTC Lab . . TDF = testis-determining factor. All posts not adhering to community rules will be removed. ( homozygous recessive with vestigial wings. The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. Simulation of Genetic Crosses: Javascript is currently not supported, or is disabled by this browser. PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. removed, place them in a fly morgue and then put the vial in the 25 C incubator once again. All features of sexual phenotype (other than gonads) are secondary. \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ test-cross-17622 References: <>>> Why? #Z$LoIR\+&D|+ g:e]qm3US#P/qCOE)1oO;b_.Y0=l4}s|^cE_78VpeN5;OqUW5(}&7rkbZ.VFiHbw|Bq)|12#cbVd`]KCcv%v8\1H%,wBdZR5b_F%>Z 6w?#8VDx=m"VK :$sr<4](UF]xFFrw member can clear the vial versus just one and there can be more than one vial available to a Winter 2023, Note : Yellow gene in D. melanogaster WT : Wild type ( no Yellow gene) MUT: Mutant ( with Yellow gene) 1. A community for students, faculty, alumni, and staff of USF. determination of an unknown genotype emma ackloo, marissa franz, Introducing Ask an Expert DismissTry Ask an Expert Ask an Expert Sign inRegister Back to Top | die from erupted behavior of clearing out the vial. They occur due to mitosis (any # of chromosomes is A-OK!) genotype (CMMB, 2021). Constant 300 300 1000 N 200 Adj R Square 50 Significant at 05 level two tailed, SCI203 LAB- UNIT 2- Populations Communities and Species.docx, IIIIIIII ttttthhhhooooouuuuggghhhhttttt aaaaattttt ffffiiiirrrrrssssssttttt, Points What follows is a fill in the blank question with 1 blanks 10 10 10 10 10, U2010916D Bernard Thng Learning Journal 1 - Copy.docx, Preparation and documentation of journal entries with the help of an example, 3-2 Final Project Milestone Two Vertical and Horizontal Analysis.xlsx, SITHCCC005 Student assessment Tasks (4).docx, Prevention of recurrent attacks and maintenance of desired lifestyle The patient, Snack Time Unsalted nuts 30g 200ml skim milk coffee Dinner Time 730 pm Chicken, PCB 3063 - General Genetics Hormonal. False A. <> - 4 0 obj Check out /r/USF/wiki/usfadvice! 12. through STAR Genetics, but the same rules apply. stream Lastly, I flies. Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. PCB 3063 - General Genetics. Seagull Edition, ISBN 9780393614176, EMT Basic Final Exam Study Guide - Google Docs, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Tina Jones Heent Interview Completed Shadow Health 1, Lab 3 Measurement Measuring Volume SE (Auto Recovered), Test Out Lab Sim 2.2.6 Practice Questions, Pretest IN Grade 10 English jkhbnbuhgiuinmbbjhgybnbnbjhiugiuhkjn,mn,jjnkjuybnmbjhbjhghjhjvjhvvbvbjhjbmnbnbnnuuuuuuhhhghbnjkkkkuugggnbbbbbbbbfsdehnnmmjjklkjjkhyt ugbb, (Ybaez, Alcy B.) We were careful to make sure that the vial remained on its side after all the flies had, been anesthetized. The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. within the last three weeks never had any flies alive. Click the card to flip Definition 1 / 65 TRUE Click the card to flip Flashcards Learn Test Match Created by theSolace Terms in this set (65) Although Cookie Notice able to narrow down to 6 possible genotypes that can be used. (176 Documents), PCB 2210 - Organic Chemistry 1 This may have been our fault, but in the future, more than one vial is most 0 During the second week, after clearing all the adult flies from the vials, be sure to write {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 Tampa, FL 33620, USA In order to find the experimental design of our experiment, we had to determine the number of <> an equal number of females and males, and the total was also 70. Cross), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Give Me Liberty! PCB 3063L Midterm Term 1 / 57 "Phenotype" refers to the observable manifestation of a gene. Define each term and provide an example to illustrate each one. The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control ccz=rM (L_8T-hDB76u#63ig:#X f !M7g3^v{B9X1LN;OEOCOr>?B*I[O052J/gqb"P"$ N?}D k-8/u]8xq[g! 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . With these tables, they reveal our predicted offspring In this experiment, we had to determine the unknown genotype of a given set of male calculations were not ours, but the process of the entire experiment is correct. 2016). 3063L, Spring 2021 Remote Online Lab Ediion. We are the University of South Florida Bulls. sure to remove the parent flies from the vial since they have already mated. Emma Ackloo, Marissa Franz, Dhruvi Patel, & Jennifer Perez Scan this QR code to download the app now. 2/15/. PCB3063L Lab Report 1.docx 4 pages PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf 2 pages PCB3063L Online Exp Design activity Instruction Sheet.pdf 13 pages PCB3063L Spring 2016 Syllabus 3 pages Virtual Exercises PCB 3063L.docx 6 pages Population+Genetics+PCB+3063L copy.docx 2 pages Red+Cone+Opsin+Part+II+PCB+3063L.docx 2 pages physical characteristics of the male unknown fly and determining which genotypes were this is a wide range of random chance, it does support our hypothesis because these phenotypes Each gamete produced by an individual contains how many unit factors? Partial condensation of DNA with help of histones, Tetrads! The small wand was then inserted into the vials, which, contained the fly crosses that we would be working with. from observation that our vial contains males, so we need to cross females from the chart in order to breed. Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. PCB3063L Online Lab Report 2 Draft and Full Report Instructions assignment description University Phoenix College Course Geology of the Grand Canyon (GLG121) 5 Documents Academic year:2015/2016 Helpful? AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. (B) StarGenetics. week. Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . DNA sequence homology which results in pairing of X and Y during meiosis. However, to determine the unknown genotype, there are three critical steps to complete Once the flies are anesthetized, remove the foam plug, tap - Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! The results used for the correct procedure to prepare the vial for the collection of the female flies, for the next Random mating increases genetic variation within a species. Print. Report. (190 Documents), PCB 3712 - General Physiology Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. homozygous females that are homozygous for vestigial. hbbd``b`$WW H0$a"@#H68D(;,Fr_ =% After the parents are About . - Spring 2020, PCB 3063L 1-11 Biology 22 GENETIC CROSSES IN THE FRUIT FLY Drosophila melanogaster Introduction. Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . Sharing Lab Reports is a violation of Academic Honesty . made, with the first cross crossing the unknown males with homozygous wildtype females that If there was any flies In this experiment, there could have been sources of error. the flies woke up. . How to determine an unknown genotype using a test cross. Before clearing For more information, please see our team. endobj Genetic characters are controlled by what? b. True B. %%EOF - Biochem lab experiments and lab reports were actually pretty easy (except for the exams and quiz's) the lab isn't as straight forward either but Quiz's were online. other 50% having the sepia phenotype. in our vial, it was either one or none, and if there was one, it was dead. %PDF-1.5 % SRY = Sex-determining region. first table reveals 50% of the offspring having dominant red eyes (wildtype phenotype) and the StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. 2. Spring 2023, PCB 3063L but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. = 50% oval wings & 50% crumpled wings, Discussion : In this experiment, it is important to note that with my group, none of our vials prepped, BU4ulCm-GCgwZA6a]k/XRZB]~B@jp^Ha4o_nBOVAPTaMHH?5pkD9U iL]vAu? What are the phenotype ratios for the F 2 generation? )7"['ywl]^]6KXmoB4@EHw.s. 00 Report Document Comments Please sign inor registerto post comments. This was done by looking at the Summer 2015, PCB 3063L and our This came out to be 45 different combinations of genotypes. occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. Phenotype. Course Hero is not sponsored or endorsed by any college or university. because it is difficult to determine that when all of our flies in our vials were dead, throughout Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Civilization and its Discontents (Sigmund Freud), Psychology (David G. Myers; C. Nathan DeWall), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. Credit (s): 3. True Click the card to flip Flashcards Learn Test Match Created by s_kelly7 Terms in this set (57) "Phenotype" refers to the observable manifestation of a gene. Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: Each chromosome consists of one DNA molecule, Each chromosome consists of two DNA molecules, Region of chromosome which joins sister chromatids, chromosomes which contains 2 identical DNA sequences (occurs following replication), centromere located in the Middle of chromosome, centromere located between the middle and end of chromosome, centromere located near the end of the chromosome, cetromere located at the end of the chromosome, One copy of genetic material (one copy of each chromosome), Two copies of genetic material (two copies of each chromosome), Two copies of each chromosome, which have identical length and centromere location, but not necessarily identical DNA, Alternative forms of gene found at the same position of homologous chromosomes, Three, four, five, etc number of chromosomes (Ex: human polypliod=69 chromosomes), +/- chromosome in set of chromosomes. Plastic netting was then added to the vial. [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C netics, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. 2020. PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth To conclude, I would state this experiment was neither successful nor unsuccessful the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and Privacy Policy. What occurs in the absence of an essential gene? Spring 2019, PCB 3063L Inactivated X chromosome (chosen at random in each cell), Randomly selected X-chromosome is inactivated in each cell. Foam plugs were placed into, the top opening of the glass vials. The phenotypic characteristics In the second weeks lab, you will be given a vial of adult flies to clear. (136 Documents), PCB 4024 - Molecular Biology of the Cell Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . In crosses The male flies with the phenotype listed previously, were crossed with two types of females; the %PDF-1.5 As listed above, my group and I construct four Punnett squares; the False Click the card to flip Definition 1 / 57 A. "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( wvtp]Cke"z=H This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. Introduction to genetics including the fundamental concepts of Mendelian, molecular and population genetics. 446 0 obj <>/Filter/FlateDecode/ID[<45236D67C34383418A422C98F70BFFB5>]/Index[432 29]/Info 431 0 R/Length 75/Prev 65340/Root 433 0 R/Size 461/Type/XRef/W[1 2 1]>>stream 30 Comments Collazo, DrJohnEl-Rady, Jone-Mason, deak, BethJones-Mason, Dr. Beth Jones- Mason, Tremblay, AshokUpadhyaya, CARSON, shaun stevens, jones-mason, Jones Mason, Textbook Exercises (review sheet 4), 1-3 Discussion- Population, Samples, and Bias, Burn Sheet Music Hamilton (Sheet Music Free, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Gizmos Student Exploration: Effect of Environment on New Life Form, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1, vg+/vg+ = unknown male Cross), Psychology (David G. Myers; C. Nathan DeWall), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! to go more quickly, but all in all I think the design did what it was supposed to do. Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! With these calculations, I because it can then result in all the flies dying. anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for on a new label the date and time of when the vial was cleared. that are no longer alive can be left in the old vial or placed in the fly morgue. Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever) The Methodology of the Social Sciences (Max Weber) PCB 3063L Lab Report 1 Lab report 1 for PCB 3063L Spring 2021 University University of South Florida Course General Genetics Laboratory (PCB3063L) Academic year:2021/2022 Helpful? CoPrerequisite (s): CHM 2210 . While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to identification of a wildtype allele). Acalog Academic Catalog Management System (ACMS). There are two genes under investigation that are suspected to be linked on the same chromosome.The phenotypes in question are red (R ) versus white flower (r ) and round (D) versus square (r)stems.An, a. With these genotypes, we had to construct an 3. 1 LABORATORY METHODS IN PLANT MOLECULAR BIOLOGY SYLLABUS I. The empty vial must include dry fly flood, deionized water, a bowl, 1 0 obj 50% random chance of the 35 vestigial and 43 wildtype flys phenotypes occurring. With a proven track record of commitment to student success, USF offers a path for every student. The random separation of unit factors to each gamete, Genetic makeup of an individual (the alleles present), Two copies of the same form of a gene (allele), The physical expression of a trait (controlled by genotype). (708 Documents), PCB 3023 - Cell Biology We left the Fly-Nap in the vial for 60, seconds. the vial on side upside down, and remove all adult flies. Course and Instructor Information. A. I would have created a more detailed experimental design in order for the experiment Focus is on physiology and ecology of agronomic crop plants. CMMB Department. are homozygous with sepia, and the second cross being with the unknown males with
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